Where a label calorie content statement is required or simply desirable, it may be calculated from the same proximate analysis data used for setting guarantees. Unlike guarantees, it is NOT declared as a minimum or a maximum, but as an average based on multiple proximate analysis data.
The calorie content of a food is dependent on the amounts of crude protein, crude fat, and carbohydrate in the product. Carbohydrates are not measured directly, but can be estimated by calculating the “nitrogen-free extract” (or NFE) in the product. This is determined simply by subtracting the average of each of the other components (percent crude protein, crude fat, crude fiber, moisture AND ash) from 100 [see the following example].
The next step is to multiply each of the average percentages for the calorie-containing nutrients by the appropriate “modified Atwater” value. Protein and carbohydrate are assigned a value of 3.5. Fat is much more calorie dense, hence has a value of 8.5. The results of the three calculations are added. Then, to convert the answer to kcal/kg (the units required on the label), the sum is multiplied by 10.
Some lab reports include calorie values. However, this information is only useful and can be used in lieu of the above calculations if the lab is familiar with calorie calculations specifically for pet foods. A laboratory that primarily analyzes human foods will not use the same modified Atwater values in its calculations, hence will give you an inaccurate calorie content number.
Also useful information is the number of calories per treat or cup of product. First needed is the weight of a single treat or a cup of product in grams. Dividing the kcal/kg value as determined above by 1000 converts it to kcal per gram. Then, multiplying by the number of grams per treat or cup gives you the calories per treat or cup.
Example: Calculating calorie content from proximate analysis data
Batch |
Crude Protein
% AF |
Crude Fat
% AF |
Crude Fiber
% AF |
Moisture
% AF |
Ash
% AF |
Nitrogen-Free Extract
% AF |
Metabolizable Energy
kcal/kg AF |
1 |
25 |
15 |
4.5 |
7.8 |
3.2 |
|
|
2 |
26 |
14.3 |
3.2 |
8.9 |
3 |
|
|
3 |
25.2 |
13 |
4 |
8.1 |
2.9 |
|
|
4 |
23 |
16 |
3.8 |
9.1 |
3.3 |
|
|
Average |
24.80 |
14.58 |
3.88 |
8.48 |
3.10 |
45.18 |
3688 |
Nitrogen-Free Extract = 100 – (crude protein + crude fat + crude fiber + moisture + ash)
Metabolizable Energy = [(3.5 X crude protein) + (8.5 X crude fat) + (3.5 X nitrogen-free extract)] X 10
Calorie content (ME)= 3688 kcal/kg; If a treat weighs 10 grams apiece, the calories per treat = 3688/1000 X 10 = 36.9 kcal/treat; If a food weighs 120 grams per cup, the calories per cup = 3688/1000 X 120 = 442.6 kcal/cup